Southern Air will begin a weekly all-cargo service between Miami International Airport (MIA) and Hong Kong via Anchorage from April.
The new scheduled freighter flights will be served by Boeing B777F aircraft.
MIA said that Hong Kong is its fourth largest market in Asia, responsible for more than $500m in freight annually.
In 2017, MIA’s trade with the entire Asia world region was valued at $7.8bn – a 16% increase from $6.7bn in 2016.
"We are thrilled to add Southern Air to our growing route network in Asia," said Miami-Dade Aviation director Lester Sola, who is returning this week from a business development mission in China and Japan.
"As the busiest US airport for international freight, it is especially gratifying to welcome additional cargo service from Hong Kong, the world’s busiest freight airport.”
Southern Air, a wholly owned subsidiary of Atlas Air Worldwide, Inc., will become the sixth airline at MIA with all-cargo service from Asia, joining: Asiana Airlines (Seoul); Cathay Pacific (Hong Kong); China Airlines (Taipei); Korean Air (Seoul); and Polar Air (Seoul).